Review



mutant human embryonic stem cell lines bg01v  (BresaGen Inc)

 
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 90
      Buy from Supplier

    Structured Review

    BresaGen Inc mutant human embryonic stem cell lines bg01v
    Mutant Human Embryonic Stem Cell Lines Bg01v, supplied by BresaGen Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mutant+human+embryonic+stem+cell+lines/bg01v/us11890304-62-13-14
    Average 90 stars, based on 1 article reviews
    mutant human embryonic stem cell lines bg01v - by Bioz Stars, 2026-09
    90/100 stars

    Images

    Related Articles

    Mutagenesis:

    Article Title: Methods for purifying cells derived from pluripotent stem cells
    Article Snippet: .. Also suitable are mutant human embryonic stem cell lines, such as, for example, BG01v (BresaGen, Athens, Ga.). ..



    Similar Products

    transgenic human embryonic stem cell hesc lines cdh1 mutant cell lines  (Addgene inc)
    94
    Addgene inc transgenic human embryonic stem cell hesc lines cdh1 mutant cell lines
    Figure 1. Disruption of apical-basal polarity by dominant-negative <t>CDH1</t> mutants in the Matrigel overlay culture system or by Cdc42 knockout in the developing mouse pancreas alters beta cell specification (A) Design of dominant-negative CDH1 mutants with deletions in the extracellular domain (CDH1DE) or the p120ctn-binding site (CDH1DP). mCh, internal ribosome entry site (IRES)-coupled mCh (red fluorescent protein) reporter. (B) Confocal images of S5 (day 13) differentiated cells (± 72 h Dox treatment). EZR, EZRIN, green; BCAT, beta-catenin, gray; mCh, mCherry, red. Scale bar, 50 mm. (C) Flow cytometry quantification of INS single-positive beta cells and GCG positive alpha cells in mCherry+ and mCherry subpopulations after Dox treatment (S4–S6). CDH1DE and CDH1DP mutations led to the same degree of reduction in the INS+GCG/GCG+ ratio in mCherry+ mutant cells compared with mCherry
    Transgenic Human Embryonic Stem Cell Hesc Lines Cdh1 Mutant Cell Lines, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mutant+human+embryonic+stem+cell+lines/hE-cadherin%2F%CE%94p120+(Plasmid+%2345770)/pm40056911-545-2-17
    Average 94 stars, based on 1 article reviews
    transgenic human embryonic stem cell hesc lines cdh1 mutant cell lines - by Bioz Stars, 2026-09
    94/100 stars
    		  Buy from Supplier
    mutant human embryonic stem cell lines bg01v  (BresaGen Inc)
    90
    BresaGen Inc mutant human embryonic stem cell lines bg01v
    Figure 1. Disruption of apical-basal polarity by dominant-negative <t>CDH1</t> mutants in the Matrigel overlay culture system or by Cdc42 knockout in the developing mouse pancreas alters beta cell specification (A) Design of dominant-negative CDH1 mutants with deletions in the extracellular domain (CDH1DE) or the p120ctn-binding site (CDH1DP). mCh, internal ribosome entry site (IRES)-coupled mCh (red fluorescent protein) reporter. (B) Confocal images of S5 (day 13) differentiated cells (± 72 h Dox treatment). EZR, EZRIN, green; BCAT, beta-catenin, gray; mCh, mCherry, red. Scale bar, 50 mm. (C) Flow cytometry quantification of INS single-positive beta cells and GCG positive alpha cells in mCherry+ and mCherry subpopulations after Dox treatment (S4–S6). CDH1DE and CDH1DP mutations led to the same degree of reduction in the INS+GCG/GCG+ ratio in mCherry+ mutant cells compared with mCherry
    Mutant Human Embryonic Stem Cell Lines Bg01v, supplied by BresaGen Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mutant+human+embryonic+stem+cell+lines/bg01v/us11890304-62-13-14
    Average 90 stars, based on 1 article reviews
    mutant human embryonic stem cell lines bg01v - by Bioz Stars, 2026-09
    90/100 stars
    		  Buy from Supplier
    mutant human embryonic stem cell lines, such as, for example, bg01v  (BresaGen Inc)
    90
    BresaGen Inc mutant human embryonic stem cell lines, such as, for example, bg01v
    Figure 1. Disruption of apical-basal polarity by dominant-negative <t>CDH1</t> mutants in the Matrigel overlay culture system or by Cdc42 knockout in the developing mouse pancreas alters beta cell specification (A) Design of dominant-negative CDH1 mutants with deletions in the extracellular domain (CDH1DE) or the p120ctn-binding site (CDH1DP). mCh, internal ribosome entry site (IRES)-coupled mCh (red fluorescent protein) reporter. (B) Confocal images of S5 (day 13) differentiated cells (± 72 h Dox treatment). EZR, EZRIN, green; BCAT, beta-catenin, gray; mCh, mCherry, red. Scale bar, 50 mm. (C) Flow cytometry quantification of INS single-positive beta cells and GCG positive alpha cells in mCherry+ and mCherry subpopulations after Dox treatment (S4–S6). CDH1DE and CDH1DP mutations led to the same degree of reduction in the INS+GCG/GCG+ ratio in mCherry+ mutant cells compared with mCherry
    Mutant Human Embryonic Stem Cell Lines, Such As, For Example, Bg01v, supplied by BresaGen Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mutant+human+embryonic+stem+cell+lines/bg01v/us10981974-931-13-14
    Average 90 stars, based on 1 article reviews
    mutant human embryonic stem cell lines, such as, for example, bg01v - by Bioz Stars, 2026-09
    90/100 stars
    		  Buy from Supplier
    mutant human embryonic stem cell lines bgoiv  (BresaGen Inc)
    90
    BresaGen Inc mutant human embryonic stem cell lines bgoiv
    Figure 1. Disruption of apical-basal polarity by dominant-negative <t>CDH1</t> mutants in the Matrigel overlay culture system or by Cdc42 knockout in the developing mouse pancreas alters beta cell specification (A) Design of dominant-negative CDH1 mutants with deletions in the extracellular domain (CDH1DE) or the p120ctn-binding site (CDH1DP). mCh, internal ribosome entry site (IRES)-coupled mCh (red fluorescent protein) reporter. (B) Confocal images of S5 (day 13) differentiated cells (± 72 h Dox treatment). EZR, EZRIN, green; BCAT, beta-catenin, gray; mCh, mCherry, red. Scale bar, 50 mm. (C) Flow cytometry quantification of INS single-positive beta cells and GCG positive alpha cells in mCherry+ and mCherry subpopulations after Dox treatment (S4–S6). CDH1DE and CDH1DP mutations led to the same degree of reduction in the INS+GCG/GCG+ ratio in mCherry+ mutant cells compared with mCherry
    Mutant Human Embryonic Stem Cell Lines Bgoiv, supplied by BresaGen Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mutant+human+embryonic+stem+cell+lines/mutant+human+embryonic+stem+cell+lines+bgoiv/us10323228-63-3-14
    Average 90 stars, based on 1 article reviews
    mutant human embryonic stem cell lines bgoiv - by Bioz Stars, 2026-09
    90/100 stars
    		  Buy from Supplier
    mutant human embryonic stem cell lines  (BresaGen Inc)
    90
    BresaGen Inc mutant human embryonic stem cell lines
    Figure 1. Disruption of apical-basal polarity by dominant-negative <t>CDH1</t> mutants in the Matrigel overlay culture system or by Cdc42 knockout in the developing mouse pancreas alters beta cell specification (A) Design of dominant-negative CDH1 mutants with deletions in the extracellular domain (CDH1DE) or the p120ctn-binding site (CDH1DP). mCh, internal ribosome entry site (IRES)-coupled mCh (red fluorescent protein) reporter. (B) Confocal images of S5 (day 13) differentiated cells (± 72 h Dox treatment). EZR, EZRIN, green; BCAT, beta-catenin, gray; mCh, mCherry, red. Scale bar, 50 mm. (C) Flow cytometry quantification of INS single-positive beta cells and GCG positive alpha cells in mCherry+ and mCherry subpopulations after Dox treatment (S4–S6). CDH1DE and CDH1DP mutations led to the same degree of reduction in the INS+GCG/GCG+ ratio in mCherry+ mutant cells compared with mCherry
    Mutant Human Embryonic Stem Cell Lines, supplied by BresaGen Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mutant+human+embryonic+stem+cell+lines/mutant+human+embryonic+stem+cell+lines/us09969981-260-3-14
    Average 90 stars, based on 1 article reviews
    mutant human embryonic stem cell lines - by Bioz Stars, 2026-09
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    Figure 1. Disruption of apical-basal polarity by dominant-negative CDH1 mutants in the Matrigel overlay culture system or by Cdc42 knockout in the developing mouse pancreas alters beta cell specification (A) Design of dominant-negative CDH1 mutants with deletions in the extracellular domain (CDH1DE) or the p120ctn-binding site (CDH1DP). mCh, internal ribosome entry site (IRES)-coupled mCh (red fluorescent protein) reporter. (B) Confocal images of S5 (day 13) differentiated cells (± 72 h Dox treatment). EZR, EZRIN, green; BCAT, beta-catenin, gray; mCh, mCherry, red. Scale bar, 50 mm. (C) Flow cytometry quantification of INS single-positive beta cells and GCG positive alpha cells in mCherry+ and mCherry subpopulations after Dox treatment (S4–S6). CDH1DE and CDH1DP mutations led to the same degree of reduction in the INS+GCG/GCG+ ratio in mCherry+ mutant cells compared with mCherry

    Journal: Developmental cell

    Article Title: Pancreatic alpha and beta cell fate choice is directed by apical-basal polarity dynamics.

    doi: 10.1016/j.devcel.2025.02.008

    Figure Lengend Snippet: Figure 1. Disruption of apical-basal polarity by dominant-negative CDH1 mutants in the Matrigel overlay culture system or by Cdc42 knockout in the developing mouse pancreas alters beta cell specification (A) Design of dominant-negative CDH1 mutants with deletions in the extracellular domain (CDH1DE) or the p120ctn-binding site (CDH1DP). mCh, internal ribosome entry site (IRES)-coupled mCh (red fluorescent protein) reporter. (B) Confocal images of S5 (day 13) differentiated cells (± 72 h Dox treatment). EZR, EZRIN, green; BCAT, beta-catenin, gray; mCh, mCherry, red. Scale bar, 50 mm. (C) Flow cytometry quantification of INS single-positive beta cells and GCG positive alpha cells in mCherry+ and mCherry subpopulations after Dox treatment (S4–S6). CDH1DE and CDH1DP mutations led to the same degree of reduction in the INS+GCG/GCG+ ratio in mCherry+ mutant cells compared with mCherry

    Article Snippet: Generation of transgenic Human embryonic stem cell (hESC) lines CDH1 mutant cell lines: The mutant CDH1 sequences27 (Addgene #45770 and #45773) were cloned into the piggyBac destination vector PB-TAC-ERN (Addgene #80475) for doxycycline induction and mCherry co-expression using Gateway technology (Thermo Fisher).

    Techniques: Disruption, Dominant Negative Mutation, Knock-Out, Binding Assay, Flow Cytometry, Mutagenesis